Abstract：Soluble protein of Fusarium oxysporium and exoantigen was injected into rabbit as antigen，tiserum with high titer was acquired，and IgG was isolated and purified.Indirect ELISA was established to detect cucumber Fusarium wilt by optimizing ELISA reactive condition.This method could test with very high sensitivity.The test results for specialities showed that besides Fusarium solani（Mart.）App.et Wr.，the IgG didn’t have any cross reaction with other pathogens in cucumber.Owing to the fact that Fusarium wilt caused by Fusarium oxysporium and root rot by Fusarium solani（Mart.）App.et Wr.use the same pesticide，this method has good practicability and with distinctive effect in the fields.Cucumbers showed early symptom were all detected positive.This means the detection with very high accuracy.
收稿日期: 2008-03-31
引用本文:
王艺凯 石延霞 李宝聚 陈红漫. 黄瓜枯萎病快速检测技术研究[J]. 中国蔬菜, 2008, 1(11): 18-23.
Wang Yikai;Shi Yanxia;Li Baoju;et al.. Studies on Identification and Expeditious Detection of Cucumber Fusarium Wilt. , 2008, 1(11): 18-23.
