Abstract：A pair of oligonucleotide primers was designed from conserved domain of EIN3 gene family.PCR amplifications were performed on different sex genotype cucumber DNA template and produced 6 fragments of 725 bp，respectively.After cloning and sequencing on CsEIN3 of different sex genotype cucumbers，BLAST and DNAMAN analysis found 14 single nucleotide polymorphisms（SNPs）in coding region，about one average 51 bp，selection rating 1.96 %，3 mutation in enzyme digested site，eight SNPs mutation cause amino acid sequence variation.One set of stable RFLP-BfmⅠenzyme digested reaction system was established.By digesting DNA from different sex genotypes，a specific enzyme digest band in WI1983H was found.
收稿日期: 2008-06-15
引用本文:
刘晓虹 陈惠明 许 亮 许 勇 张海英 卢向阳. 不同性别黄瓜EIN3基因片段克隆及序列分析[J]. 中国蔬菜, 2008, 1(9): 10-14.
Liu Xiaohong;Chen Huiming;Xu liang;et al.. Cloning and Sequence Analysis of EIN3 Gene from Cucumis sativus with Different Sex Expression. , 2008, 1(9): 10-14.
