In this study,it established and optimized SSR detection system including

reaction components,amplified program,by using cabbage inbred lines 15R（‘Nanbao’）and

14S(‘Beiheidapingtou’).A stable SSR-PCR system was followed:10.00 μL reaction solution

contained 1×Buffer，50.00 μmol·L^-1 dNTPs，0.40 U Taq enzymes，0.40 μmol·L^-1 SSR primers

pairs，1.00 μL DNA(60.00 ng·μL^-1).PCR amplifying program was divided into two steps,after

one denaturing step of 5 min at 94 ℃,DNA was denatured for 45 s at 94 ℃,the annealing

temperature was 60 ℃ and dropped 0.5 ℃ for each cycle,until a final annealing temperature

of 50 ℃ was reached.For the last 15 cycles of the amplification,an annealing temperature

of 55 ℃ was employed.When all cycles were finished,amplification ended for 10 min at 72 ℃

.The polyacrylamide gel electrophoresis was used in the detection of PCR amplification.