Abstract：26 different strains of Botrytis cinerea Pers were used to identify the resistance of two pure lines of cucumber to gray mold by the inoculation method of mycelial plugs at seedling stage and in vitro leaves respectively.Results showed that the average area of disease spots in leaves infected by 26 strains were between 0 to 556.15 mm2，showing that the difference of pathogenicity was very significant.Resistance differences existed between two genotypes of cucumber inoculated by different strains.Analysis of gray correlation degree proved that the identification result of the inoculation method of in vitro leaves was basically coincidence with those of the inoculation method at seedling stage by certain strains.
