Studies on Plant Regeneration and Ploidy Identification of Microspore Embryiods in Broccoli(Brassica oleracea L.var.italica Plenck)
WANG Chun-li,WANG Tao-tao,ZHANG Yu-yang,LI Han-xia*
Huazhong Branch of National for Vegetable Improvement Center,College of Horticulture & Forsetry Sciences,Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,Hubei,China
Taking broccoli(Brassica oleracea L.var.italica Plenck)variety‘Qingnong 70’and‘Qingnong 80’as experimental materials,this paper studies on plant regeneration factors of microspore embryoids.The results showed that the embryoids age(30-35 days)was optimal to plant regeneration,and the regeneration frequency exceeded 75 %,while it decreased significantly when the embryoids age was over 40 days.The regeneration frequency reached the highest(up to 60 %-70 %)when agar in the medium was 1.2 %.Most of embryoids could not produce plantlets directly,subculture was needed,and this treatment helped to differentiate much more plantlets.The optimal media for subculture was MS+3 % sucrose+1.0 % agar.About half of the regenerated plants were haploid,30 %-35 % plants were double haploid,and the rest were a few tetraploid or chimeras.
