Cloning of a cDNA Fragment of Acid Invertase Gene from Tomato Fruit
Yu Xiyan 1; Zhao Shuangyi 2; He Qiwei 3; et al.
1College of Horticulture; Agriculture University; Tai′an 270118; 2College of Life Sciences; Shandong University; 3Vegetable Institute; Shandong Academy of Agricultural Sciences
Abstract：PCR primers were designed based on the consensus domain of some acid invertase genes in GenBank. A cDNA fragment was amplified from the total RNA isolated from toamto fruit via RT PCR and cloned into pMD18 T vector. The determined sequence showed that the amino acid sequence is 99% homologous to that of the corresponding region of acid invertase gene (Access No. M81081) in GenBank. This confirmed that we cloned a cDNA fragment from tomato fruit successfully and its access number is confirmed as AF490530 i...
收稿日期: 2002-03-28
引用本文:
于喜艳;赵双宜;何启伟;侯丽霞;. 番茄果实酸性转化酶基因cDNA片段的克隆[J]. 中国蔬菜, 2002, 1(6): 9-11.
Yu Xiyan;Zhao Shuangyi;He Qiwei;et al.. Cloning of a cDNA Fragment of Acid Invertase Gene from Tomato Fruit. , 2002, 1(6): 9-11.
