In order to get consistent reproducible results, the system for identifying physiologic races of Fusarium oxyporum f. sp. niveum（FON）is suggested, in which the important factors influencing the results were determined based on the previous study results. The test was conducted and demonstrated that physiologic race 0, 1, and 2 of FON could be identified according to the system. It indicates that the system can be used as standards for identifying a physiologic race of FON. The system can also be used for conducting studies on physiologic race differentiation or pathogenicity of FON, and for screening watermelon breeding material for resistance to Fusarium wilt.