Abstract：14 cucumber（Cucumis sativus L.）genotypes were screened for this study．The coefficient analysis and path analysis were carried out between the low-nitrogen tolerance index and all interrelated indexes．It was found that the chlorophyl b content was the ideal index for screening low-nitrogen tolerance in cucumber genotypes，and that D0328 and D0422 had the highest and lowest content of chlorophyl b，respectively．Then D0422×D0328 were chosen to make the joint segregation analysis of 6 generations，which ranged from P1 to F2．The results showed that low-nitrogen tolerance of cucumber was a kind of quantitative trait；and it matched the AD model while the additive effect played the main role．Both the broad heritability and narrow heritability were high，so the inheritance of cucumber low-nitrogen tolerance trait was hardly impacted by environmental factors.
