Abstract：P2300-pa-8e gene was originated from Bt185，which exists in insect resistance transcription factor．In this study，P2300-pa-8e gene was transferred into the potato（Solanum tuberosum L.）cultivars，‘Zaodabai’and‘Kexin No.18’by agro-bacterium-mediated transformation，and influence factors of genetic transformation were optimized．Microtuber discs were used as explants．19 resistant plants of‘Zaodabai’and 21 resistant plants of‘Kexin No.18’were obtained．Resistant plants were blot analyzed by PCR detection，of which 5 and 9 plants were positive．And these resistand plants were analyzed by Southern-blot，of which 4 and 6 plants had a positive amplification．Southern blot showed that single copies of the P2300-pa-8e gene were inserted into the genome of the transgenic plants．By the result of insect-resistant experiment，the transgenic materials showed resistance to coleopteran pests Holotrichia parallela and the larvae of Holotrichia oblita（Fald.）．
