中国蔬菜 ›› 2012, Vol. 1 ›› Issue (10): 75-79.

• 试验研究 • 上一篇    下一篇

济南地区倒瓤西瓜CGMMV的检测

刘颖,彭斌,杨晓红,李向东,古勤生   

  1. 1南方山地园艺学教育部重点实验室,西南大学园艺园林学院,重庆 400715|2河南省果树瓜类生物学重点实验室,中国农业科学院郑州果树研究所,河南郑州 450009|3山东农业大学植物保护学院,山东泰安 271000
  • 收稿日期:2012-02-09 出版日期:2012-05-15 发布日期:2012-03-06
  • 通讯作者: 杨晓红,教授,博士生导师,专业方向:应用微生物学,E-mail:yangxh2@swu.edu.cn|古勤生,研究员,博士生导师,专业方向:分子植物病理学,E-mail:guqsh@126.com
  • 作者简介:刘颖,女,硕士研究生,专业方向:微生物学与植物营养,E-mail:jsliuying@163.com
  • 基金资助:

    国家现代农业产业技术体系建设专项(CARS-26-13)

Detection of Cucumber Green Mottle Mosaic Virus of Watermelon Blood Flesh in Jinan Area

LIU Ying1,2,PENG Bin2,YANG Xiao-hong1*,LI Xiang-dong3,GU Qin-sheng2*   

  1. 1Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China|2Biological Technology Center,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China|3College of Plant Protection,Shandong Agricultural University,Tai’an 271000,Shandong,China
  • Received:2012-02-09 Online:2012-05-15 Published:2012-03-06

摘要: 山东省济南地区2011年嫁接西瓜严重发生倒瓤,通过实地调查,初步判断为病毒侵染引起,经对采集的80份西瓜病样进行ELISA(Enzyme-Linked Immuno-Sorbnent Assay)和RT-PCR(Reverse Transcription Polymerase Chain Reaction)验证。结果表明:经ELISA检测80份西瓜样本中有70份感染了黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV),证明山东省济南地区嫁接西瓜严重发生倒瓤是由CGMMV引起的。

关键词: 西瓜, 倒瓤, 黄瓜绿斑驳花叶病毒, ELISA, RT-PCR

Abstract: The blood flesh of watermelon〔Citrullus lanatus(Thunb.)Matsum. et Nakai〕out broke on grafted watermelon in Jinan area,Shandong Province in 2011.The viral pathogen was suspected as its cause after survey in fields.Eighty samples of grafted watermelon leaves and fruits with viral symptoms and non-symptom collected from Jinan area,Shandong Province were detected by ELISA and RT-PCR.The results showed that 70 of these 80 samples were infected by Cucumber green mottle mosaic virus,preliminary confirming that CGMMV was the cause of blood flesh of watermelon in Jinan area,Shandong Province.

Key words: Watermelon, Blood flesh, CGMMV, ELISA, RT-PCR

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