Determination of Silencing Suppressor of Tomato Yellow Leaf Curl Virus by Agroinfiltration
ZHANG Yu-yang1，YEDIDYA Gafni2，LI Han-xia1*
1Key Laboratory of Horticultural Plant Biology，Ministry of Eduction，Huazhong Agricultural University，Wuhan 430070，Hubei，China|2Institute of Plant Sciences，Agricultural Research Organization of Israel，Bet Dagan 50250，Israel
Abstract：We described the utilization of agroinfiltration to screen and verify the silencing suppressor，V2，of Tomato yellow leaf curl virus（TYLCV）．The target gene together with the GFP gene was transiently co-expressed in leaves of Nicotiana benthamiana by agroinfiltrations．In all tests of agroinfiltrations，the GFP showed high expression at 2 days post inoculation（dpi），showing the infiltration and expression was successful．On the leaves co-infected with the BV1 gene of Watermelon chlorotic stunt virus or the empty vector pBIN，the GFP fluorescence almost disappeared at 7 dpi due to silencing mechanism of the host plant．The leaves co-infected with V2，showed continuously high expression of GFP，indicating that V2 is the silencing suppressor of TYLCV．Agroinfiltration can be utilized as potent tool for screening the silencing suppressor of plant virus．
