Identification and Analysis of Mutant Phenotype Tomato Inbred Line TTD302AInduced with EMS
To create new tomato（Solanum lycopersicum L.）germplasm applied both to genetic modified breeding and functional genomics research on tomato，this study treated the seeds of TTD302A with 0.7% EMS for 8 h，then washed them by flowing water. M2 seeds were harvested from each plant independently in M1 generation. Afterwards，the agronomic traits and biological characters of each plant were both investigated and recorded in M2 generation at seedling stage，flowering fruit period and mature period，respectively. The leaves DNA of all mutation plants were extracted，of which 15 materials expressing typical variation characteristics were selected for SSR molecular marker detection. Finally we harvested M3 seeds from single plant expressing character mutation separately. Through observation on M2 group characters，we found 373 mutant characters and 297 mutant plants in total. The rate of all M2 phenotype mutation added up to 7.1%. It could be found that the mutation population contained abundant mutation types appearing in leaf，floral organ，fruit and plant. Their mutation rates were 1.5%，2.8%，1.3%，2.4%，respectively. The results of SSR analysis indicated that 9 materials had mutation in DNA level.
Abstract：To create new tomato（Solanum lycopersicum L.）germplasm applied both to genetic modified breeding and functional genomics research on tomato，this study treated the seeds of TTD302A with 0.7% EMS for 8 h，then washed them by flowing water. M2 seeds were harvested from each plant independently in M1 generation. Afterwards，the agronomic traits and biological characters of each plant were both investigated and recorded in M2
generation at seedling stage，flowering fruit period and mature period，respectively. The leaves DNA of all mutation plants were extracted，of which 15 materials expressing typical variation characteristics were selected for SSR molecular marker detection. Finally we harvested M3 seeds from single plant expressing character mutation separately. Through observation on M2 group characters，we found 373 mutant characters and 297 mutant plants in total. The rate of all M2 phenotype mutation added up to 7.1%. It could be found that the mutation population contained abundant mutation types appearing in leaf，floral organ，fruit and plant. Their mutation rates were 1.5%，2.8%，1.3%，2.4%，respectively. The results of SSR analysis indicated that 9 materials had mutation in DNA level.
