Abstract：Taking Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕‘Jihong 82’，some of #534DH and #438 population as testing material，studies the effect of mechanical extraction and artificial extrusion on micro-spore viability and extraction quantity．The result indicates that mechanical extraction can replace the artificial one，get multiple samples during short period of time，and rapidly adjust the concentration of microspores culture．40 suitable buds are collected in 5 mL centrilfuge tubes，3 000 r·min-1 micro-spores gained by 10 s smashing extraction are isolated in 25 mL NLN-13 medium．Thus，micro-spore concentration is insured in suitable culture scope，and successfully induced to embryogenesis．
李菲, 张淑江, 章时蕃, 张慧, 孙日飞, 张振贤. 大白菜游离小孢子培养技术高效体系的研究[J]. 中国蔬菜, 2014, 1(8): 12-.
LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei, ZHANG Zhen-Xian. Studies on High Efficient System for Microspore Culture Technology of Chinese Cabbage. , 2014, 1(8): 12-.
