Applicability Evaluation and Application of Linked Markers for Restorer-offertility in Pepper
MI Zhi-bo，ZHANG Jian-ying，WANG Ping-yong，LIU Chen，SHEN Huo-lin*
College of Agronomy and Biotechnology，China Agricultural University，Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops，Beijing 100193，China
Abstract：In order to accurately and efficiently use molecular marker assisted selection technique in selecting Restorer-of-fertility gene in pepper（Capsicum annuum L.） lines，this experiment carried out applicability test on the 11 pairs of reported Restorer-of-fertility gene linked markers with 36 pepper inbred line，whose genotype（RfRf or rfrf） has been known. The results indicated that dominant markers CRF3S1S and CRF-SCAR had the broadest applicability，and their accuracy rate were 91.67% and 88.89%，respectively. CaRf-FL-M2 had the broadest applicability among the 6 pairs of co-dominant markers，and its accuracy rate was 80.56%. In breeding practice，CRF3S1S or CRF-SCAR markers could be used to preliminarily identify pepper materials. Then one more identification could be conducted on pepper material containing Rf gene by CaRf-FL-M2 marker.
米志波, 张建盈, 王平勇, 刘辰, 沈火林. 辣椒恢复基因连锁标记的适用性评价与应用[J]. 中国蔬菜, 2015, 1(2): 25-29.
MI Zhi-Bo, ZHANG Jian-Ying, WANG Ping-Yong, LIU Chen, SHEN Huo-Lin. Applicability Evaluation and Application of Linked Markers for Restorer-offertility in Pepper. , 2015, 1(2): 25-29.
