Screening and Identification of Bio-control Agents Against Phytophthora melonis
SUN Li1， YI Tu-yong1，2，3*，SU Zhi-ping4，WANG Ya-fei1， WEI Run-jie1， DONG Jun1
（1College of Plant Protection， Hunan Agricultural University， Changsha 410128， Hunan，China； 2Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Plant Pests， Changsha 410128， Hunan，China； 3Key Laboratory of Control and Utilization of Plant Disease， College of Hunan Province， Changsha 410128， Hunan，China； 4Yantai Entry-exit Inspection and Quarantine Bureau，Yantai 264000，Shandong，China）
Abstract：In order to screen effective antagonistic agents to Phytophthora melonis，269 strains were isolated from collected soil in Hunan and Beijing with gradient dilution method. 2 strains showed obvious antagonistic effects to Phytophthora melonis by using agar plate dual culture，and strain X54 and strain P3 were finally screened out. The diluted 5 times filtration showed their inhibition rates were over 70%. Through observation of culture morphological characteristics，physiological and biochemical experiment，and molecular identification of 16S rDNA，we found that actinomycete X54 was Streptomyces virginiae and bacterial P3 was Bacillus licheniformis.
