Taking tomato 13485（containing Cf-5，Tm-1 and Mi-1 gene）as female parent， we crossed it with advanced tomato inbred line 13065（containing Ty-2，Ty-3，I-2，Ph-2 and Ph-3 gene）and advanced tomato inbred line 13337（containing storage tolerant gene rin） as male parents， respectively by pyramiding technology. Then we identified the genotype of 1 057 plants from their F2 segregation populations by molecular marker assistant selection（MAS） technology. We got 19 plants with resistance to 5 diseases， 30 plants with resistance to 4 diseases， and 20 plants with resistance to 3 diseases and containing rin gene. We kept those single plants with good field performance as reserve seeds， and will carry out selection afterwards. At the same time， we tested and verified the accuracy of molecular marker by inoculation identification. The result indicated that the goodness of fit by inoculation identification and molecular identification was over 80%.