中国蔬菜 ›› 2008, Vol. 1 ›› Issue (1): 20-22.

• 试验研究 • 上一篇    下一篇

甘蓝类蔬菜亲缘关系的RAPD初步分析

田 源 王 超   

  1. 东北农业大学园艺学院 150030
  • 收稿日期:2007-06-05 修回日期:2007-10-20 出版日期:2008-01-18 发布日期:2008-01-18

Initial Analysis on Genetic Relationship of Brassica oleracea L.by RAPD

Tian Yuan,Wang Chao   

  1. (College of Horticulture,Northeast Agricultural University,Harbin 150030)
  • Received:2007-06-05 Revised:2007-10-20 Online:2008-01-18 Published:2008-01-18

摘要: 利用RAPD标记从DNA水平上对30份甘蓝类蔬菜材料进行亲缘关系和遗传多样性分析,从100对引物中选出10对扩增效果较好的引物,用其扩增出90条带,其中多态条带72条,多态率为80.0 %。对RAPD结果进行UPGMA分析,结果表明,在相似系数0.85处可以清楚的将它们划分为6组,并且从分子生物学的角度分析了它们之间的亲缘关系。

关键词: 甘蓝类蔬菜, RAPD, 遗传多样性, 亲缘关系

Abstract: RAPD analysis on genetic relationship and genetic diversity of 30 samples of Brassica oleracea L.was conducted.Ten primers were selected from 100 primers to amplify 30 samples,and a total number of 90 bands wereamplified,among which 72(80.0 %)were polymorphic.The results of RAPD were analyzed by UPGMA clustering.The result showed that all samples could be divided into 6 groups with the similarity coefficient of 0.85,and their genetic relationship was analyzed.