Abstract:An efficient plant regeneration system was established through optimization of hormone combination,genotype,producing of adventitious bud in celery culture.The best media for callus induction and subculture were MS medium supplemented with 2,4- D 1.0 mg·L~ -1 +KT 1.0 mg·L~ -1 ,2,4-D 0.5 mg·L~ -1 +BA 0.2 mg·L~ -1 + CH 500 mg·L~ -1 +4 % mannitol.Genotype was an important factor for callus and somatic embryos induction.60 % Callus could be transfered from non-embryonic to semi-embryonic and embryonic callus.On...
收稿日期: 2006-05-10
引用本文:
韩清霞;沈火林;朱鑫;陈蓉;杨辉;杨学妍;. 芹菜胚性愈伤的诱导及高频植株再生体系的建立[J]. 中国蔬菜, 2006, 1(11): 6-8.
Han Qingxia;Shen Huolin;Zhu Xin;et al.. Embryonic Callus Induction and Plant High Efficient Regeneration System from Celery(Apium graveolens L.). , 2006, 1(11): 6-8.