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Cloning of the Genes Resistant to Phytophthore Capsici in Pepper by Using the Differential Display Technique |
Yi Tuyong; Zhang Baoxi; Xie Bingyan; et al. |
College of Bio-safety Science and Technology; Hunan Agricultural University; Changsha 410128; Institute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081 |
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Abstract We cloned two differentially expressed DNA fragments by using DDRT-PCR of the total RNA from the leaves of two pepper lines inoculated with Phytophthora capsici.We sequenced the fragments after cloning them into a T-vector and BLAST analysis indicated they came from two novel genes.The sequences are available from GenBank as accession AY497910 and AY497911.
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Received: 10 January 2005
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