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Transformation of γ-thionin THI gene into muskmelon (Cucumis melo L.) by mediated Agrobacterium tumefaciens |
XIAO Shou-hua, QIAO Wei-hua, ZHANG Yue-min, et al |
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Abstract Antifungal γ-thionin gene THI which had been cloned from Triticum aestivum and driven by green specific promoter (PNZIP) was transferred into muskmelon by Agrobacterium tumefaciens. Transgenic melon lines carried with both γ-THI gene and npt-Ⅱ gene were assessed by PCR and Northern blotting. The reslults showed that the foreign genes were proved to be transferred into the genome of muskmelon and expressed in leaf tissue of the transgenic plants.
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Received: 16 April 2008
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