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Identification of CMV and Screening for CMV Resistance of Pepper in Guangzhou |
Sun Xiudong1,2,Lei Jianjun2,Zhou Shumei3,et al. |
College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018 |
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Abstract An isolate of Cucumber mosaic virus was obtained from pepper in Guangzhou.The coat protein(CP)gene was amplified by RT-PCR using the genomic RNA as template and a pair of primers specific to CMV.Results of sequence analysis showed that the isolate was identified as an isolate of CMV,belonging to the subgroupⅠ.Forty-five pepper breeding lines was inoculated with CMV in seedling period.The results showed that 4 lines were resistant to CMV,and 20 lines were middle resistant.Five lines were selected possessing both CMV resistance and excellent agronomic characteristics.
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Received: 17 August 2007
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