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Cloning and Sequence Analysis of EIN3 Gene from Cucumis sativus with Different Sex Expression |
Liu Xiaohong1,Chen Huiming1,2,Xu liang2,et al. |
1Hunan Vegetable Research Institute,Changsha 410125 |
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Abstract A pair of oligonucleotide primers was designed from conserved domain of EIN3 gene family.PCR amplifications were performed on different sex genotype cucumber DNA template and produced 6 fragments of 725 bp,respectively.After cloning and sequencing on CsEIN3 of different sex genotype cucumbers,BLAST and DNAMAN analysis found 14 single nucleotide polymorphisms(SNPs)in coding region,about one average 51 bp,selection rating 1.96 %,3 mutation in enzyme digested site,eight SNPs mutation cause amino acid sequence variation.One set of stable RFLP-BfmⅠenzyme digested reaction system was established.By digesting DNA from different sex genotypes,a specific enzyme digest band in WI1983H was found.
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Received: 15 June 2008
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