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| Optimization of Technology for Embryo Rescue in Distant Hybridization between Cabbage and Radish |
| HU Xin-min1,2,LI Xi-xiang2*,LIANG Yan1,FANG Zhi-yuan2,SHEN Di2,QIU Yang2 |
| 1College of Horticulture,Northwest A&F University,Yanglin 712100,Shaanxi,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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Abstract The distant hybridization between dominant male sterile line of cabbage‘C08-11’and radish inbred line‘Q07-12’was made in this experiment.Through in vitro culture of cross-bred ovary and ovule at different developmental stages,the appropriate sampling time，culture medium and additives for the embryo rescue in distant hybridization between cabbage and radish were studied.The results were as follows:ovule culture was obviously better than ovary culture.By ovary culture only 3 hybrid seedlings were gotten and the suitable time for detaching ovary was 9 days after pollination.Adding growth regulators（6-BA，NAA）,casein acids hydrolysate and activated charcoal in basic medium B5 was necessary for ovary culture.By ovule culture，29 hybrid seedlings were gotten and the appropriate time for detaching ovule was respectively 14 days after bud pollination and 16 days after florescence pollination.Whether adding growth regulator or not depended on the sampling period and culture medium for ovule culture.
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Received: 09 February 2009
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