|
|
|
| Acquirement of Leaf Mutants from Brassica Allosextuple Species |
| ZHANG De-shuang,ZHANG Feng-lan,YU Yang-jun,ZHAO Xiu-yun,YU Shuan-cang,XU Jia-bing |
| Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China |
|
|
|
|
Abstract CGMCC № 2553 were cultivated via isolated microspore culture and embryoids of different stages were obtained.DH regeneration plants were obtained by subculture.After DH plants were set at low temperature treatment situation for 15-20 days,they would grow in greenhouse or they were directly grew in greenhouse with naturally lower temperature for 30-40 days.Results showed 172 DH plants in 2006，230 DH plants in 2007 were obtained，275 plants had been domesticated in 2008.Some DH regeneration plants could transform into leaf mutants.From 2006 to 2007,8 wrinkledness leaf mutants were gotten.The highest rate of getting mutants was 2.29 %.2 mutant plants had gotten seeds.The leaf mutants mainly showed wrinkledness in the surface of preceding leaves at early stage.Then the wrinkle leaves could transfer to normal ones and some also keep the wrinkledness later with their growth.Further studies showed some DH plants could generate two or more than two stems in which wrinkledness mutant and wild type often burgeoned at the same parent.The mutants had the same characteristics as wild types except for the leaf wrinkledness,therefore they were the better materials for studying the wrinkledness characteristic.The finding is useful in studying,locating and cloning the wrinkledness gene(s)linked to leaf mutants of Brassica allosextuple CGMCC № 2553 DH lines in the future.
|
|
Received: 16 November 2008
|
|
|
|
|
|
|
|
|
