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Studies on Identification of Eggplant Phomopsis Rot Caused by Phomopsis vexans and Its Biological Characteristics |
WU Ren-feng,YANG Shao-li,YANG De-zhi |
Wuhan Vegetable Research Institute,Wuhan 430065, Hubei, China |
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Abstract The morphological characteristics,pathogenicity and biological characteristics of the pathogens isolated from Eggplant phomopsis rot of Wuhan were studied.Large numbers of diseased eggplant samples were collected from different planting areas of Wuhan.Fifteen isolates of possible pathogenic fungi were isolated from leaves,stems and fruits of infected phomopsis rot plants.Based on the pathogenicity experiment,the results showed that the 15 isolates were the pathogens of Eggplant Phomopsis Rot disease.The morphological identification showed that 15 isolates were Phomopsis vexans.The results of studies on biological characteristics of pathoge ns showed that the optimum culture medium for mycelial growth were PDA and PSA, beneficial to spores culture medium were oats medium,the optimum temperature for mycelial growth were 25-30 ℃,and the optimum pH value were 4-7,and light promoted the growth of mycelial.The pathogen could make use of various carbon and nitrogen sources. Maltose was the best carbon source and sodium nitrate was the best nitrogen source.The lethal temperature for mycelial growth was 55 ℃ for 25 min.
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Received: 07 November 2012
Published: 28 November 2012
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