|
|
|
| Effects of Molybdenum Deficiency on Cold Resistance and Its Relationship with Antioxidant System in Cucumber Seedlings |
| CAI Huan, ZHU Shuai, NING Yu, WU Guo-Xiu, AI Xi-Zhen |
| College of Horticulture Science and Engineering， Shandong Agricultural University， State Key Laboratory of Crop Biology， Key Laboratory ofHorticultural Crop Biology and Germplasm Innovation ofAgriculture Ministry， Tai’ an 271018，Shandong， China |
|
|
|
|
Abstract In order to investigate the mechanism of molybdenum deficiency on chilling tolerance in cucumber（ Cucumis sativus L.） seedlings，this paper takes‘Jinyou No.3’ cucumber seedlings as test material，and sets up 2 treatments：molybdenum deficiency（-Mo） and molybdenum application（CK）. The low temperature treatment （day/night temperature of 8 ℃ /5 ℃） was carried out in light growth chamber. The effects of molybdenum deficiency on chilling injury index，MDA content，antioxidant enzyme activities，and osmoregulation substances of cucumber seedlings were studied. The results showed that under low temperature -Mo treatment，the electrolyte leakage（EL），chilling injury index，and MDA content of cucumber seedlings were all significantly higher than that of the CK；while the activities of superoxide dismutase（SOD），peroxidase（POD），catalase（CAT） and ascorbate peroxidase（APX），and contents of glutathione（GSH），and ascorbic acid（AsA） in cucumber seedlings were significantly lower than that of the CK. The contents of proline，soluble sugar，soluble protein and free amino acids were all lower than that of the control. These results suggest that molybdenum deficiency aggravates chilling damage to the membrane，and reduces the cold resistance of cucumber seedlings. The reduction in antioxidant abilities and osmotic self-regulation of -Motreatment are the important reasons for cold tolerance weakening of cucumber seedlings.
|
|
Received: 09 April 2014
Published: 17 November 2014
|
|
|
|
|
|
|
