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| Identification of Seedling Disease Pathogens in Greenhouse Vegetable and Screen of Fungicides |
| YANG Yu-hong，YANG Cui-rong，LING Jian，MAO Zheng-chuan，CHEN Guo-hua，XIE Bing-yan |
| （Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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Abstract Major seedling diseases of 10 vegetable species in greenhouse，including Cruciferae（Chinese cabbage，broccoli，cabbage，radish），Solanaceae（tomato，pepper，eggplant）and Cucurbitaceae（cucumber，wax gourd，pumpkin）were investigated and their pathogens were identified according to their morphological characteristics and molecular biology．The control effect of fungicides collected from different sources and against 4 main seedling diseases including damping-off，rhizoctonia disease，Fusarium wilt disease and Phytophthora blight were screened．The results showed that 736 bacteria strains were isolated from 864 seedling disease samples of 10 vegetable species．And 16 isolates were identified，among them 13 infective pathogens could generate 8-9 types of seedling diseases in various vegetable species．The most prevalent seedling diseases were traditional damping-off and rhizoctonia diseases，followed by root rot，Phytophthora blight and gray mold．The effective fungicides were screened out．32.5% Difenoconazole·azoxystrobin suspension concentrate（SC）and 2.5% fludioxonil SC had remarkable effect in controlling rhizoctonia disease．70% thiophanate methyl wettable powder（WP），36% Thiophanate-Methyl SC and 3% metalaxyl·hymexazol aqueous solutions（AS） were effective for controlling Fusarium wilt．68% metalaxyl-M mancozeb water dispersible granule（WG），25% metalaxyl-propamocarb WP，72% cymoxanil·mancozeb WP，50% dimethomorph WG，50%Fluazinam SC，64% oxadixyl·mancozeb WP and 10% cyazofamid SC had outstanding effect in controlling Phytophthora blight，50% Fluazinam SC and 64% oxadixyl·mancozeb WP，10% cyazofamid SC，72% cymoxanil·mancozeb，propamocarb hydrochloride WP，72.2% Propamocarb hydrochloride AS，50% dimethomorph WG，68% Metalaxyl-M mancozeb WG and 60% pyraclostrobin+metiram WG etc.were effective for controlling damping-off．
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Received: 05 January 2015
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