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| Studies on Quickly Testing Pakchoi Seed Vigor by Resazurin Reagent |
| LIU Chun-xiang，LI Yuan-yuan，WANG Meng，HU Pan |
（Key Laboratory of Biochemistry and Molecular Biology，Universities in Shandong Province，Weifang college，Weifang
261061，Shandong，China） |
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Abstract In order to explore simple，rapid and nondestructive method for testing pakchoi〔Brassica campestris L. ssp. chinensis（L.）Makino var.communis Tsen et Lee〕seed vigor，‘Suzhouqing’and‘Shangha iqing’were used as experimental materials under 44 ℃ condition they underwent aging treatment for 0，2，4，6 days to test their seed vigor index and color change of resazurin reagent，absorbancy，and then correlation analysis was conducted．The result showed that the variation trend of these two pakchoi varieties were the same，their germination rate，germination index and vigor index were all dramatically declined along with the increase of aging degree．The color of resazurin reagent was changed from blue to pink then to colorless．The absorbance declined sharply．The seed germination rate，the absorbancy of resazurin reagent，blue hole rate were significantly positive correlated．The correlation coefficients were over 0.96．The seed vigor index，germination index and absorbancy of resazurin reagent were significantly or highly significantly positive correlated．
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Received: 26 October 2014
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