The co-dominant AFLP marker was successfully converted into simple and applied SCAR markers.Two
special SCPM166 and SCPM66 were designed according to the sequence information of AFLP fragments.The SCAR-PCR
result showed that the resistant parent,resistant F2 plants and hybrid plants possessed 166 bp fragment,while
the susceptible parent and susceptible F2 plants did not possess any fragments by primer SCPM166.This
indicated that the SCAR marker could identify susceptible homozygous single plant.The susceptible
parent,susceptible F2 plants and hybrid plants possessed 66 bp fragment,while the resistant parent and
resistant F2 plants did not possess any fragments by primer SCPM66.This indicated that the SCAR marker could
identify homozygous resistant single plant.By joint application of these 2 dominant SCAR markers,homozygous
resistant plant and homozygous susceptible plant could be identified simultaneously.It is equivalent to a
commonly dominant SCAR marker.
Taking KCI and K2SO4 produced in Russia,Luobupu K2SO4 produced in Xinjiang and K2SO4 produced in
Kaifeng as experimental materials,the experiment was carried out to study the effects of different K sources
and applying dosage on cabbage varieties of‘Redai’and‘Moyu’.The results showed:potassium application
could increase cabbage yield,the content of protein and VC,decrease the content of nitrate.When the amount
of K application was 300 kg·hm-2,the effects of Luobupu K2SO4 was better than or close to the other K
sources.K2SO4 produced in Russia and K2SO4 produced in Kaifeng had the worst effect.Potassium application
increased‘Redai’output value by 29.9 %-33.2 %,and that of‘Moyu’by 15.8 %-20.5 %.Utilizing return
equation,it gained the best economical potassium application for‘Redai’was 354.0 kg·hm-2,the highest
output value was 98 463.4 Yuan·hm-2;that for‘Moyu’was 333.0 kg·hm-2 and the highest output value was 98
201.2 Yuan·hm-2.
Effect of exogenous allelochemical vanillic acid on soil enzyme activity and soil nutrient for
pepper seedling was studied in this article.The results showed that 4 kinds of soil enzyme activities were
increased along with the increase of vanillic acid concentration.Furthermore,polyphenol oxidase activity
increased by 13.3 %,compared to the control when vanillic acid concentration got 1×10-7 mol·L-1.The
contents of soil organic matter,available P and available K tended to decrease with the increase of vanillic
acid concentration.While the soil NO-3-N content tended to increase.Relations between the 4 soil enzyme
activities and soil organic matter content,available P and available K were significantly negative.But their
relation with soil NO-3-N contents was significantly positive or very significantly positive.
Cucumis melo L.seedlings of‘Yindi No.3’and‘Xizhoumi No.1’were used to study the effects of
different root-zone temperatures on chlorophyll content,photosynthesis and chlorophyll fluorescence
parameters by Eco-organic soilless cultivation experiments.The results indicated that chlorophyll content,net
photosynthetic rate(Pn),stomatal conductance(Gs),stomatal limitation value(Ls),transpiration rate
(Tr),chlorophyll fluorescence(F),photochemical quantum yield(Yield),photochemical quenching(qP)and non-
photochemical quenching(NPQ)were decreased with the decreasing of root-zone temperature.The decline of net
photosynthetic rate of Cucumis melo L.was caused by non-stomatal limitations under low root-zone
temperature.Inhibition of PSⅡ photochemistry activity is one of the important causes for low net
photosynthetic rate of Cucumis melo L.seedlings under low root-zone temperature.
Taking three different sweet pepper varieties as test materials,and sun shade net to stimulate
artificial low light environment,this paper studied the effect of low light on the growth of different sweet
pepper varieties.The results showed that after 85 % low light treatment,the bud stage,flowering period,the
door pepper fruit setting period,two-layer pepper fruit setting period,harvesting period and defeat seedling
stage of the three varieties were obviously delayed,compared with the control.The fresh weight of single
plant leaves decreased 43.97 %-77.26 % and the number of leaves per plant reduced 12.95 %-63.74 %.The longer
the low light treatment last,the greater the amount of reduced.Among these 3 tested varieties,J04 has the
stronger resistence to low light.Under low light conditions,its plant height,stalk thickness,number of single
plant leaves and fresh weight of single plant leaves were kept in a high value,and its number of fruit
setting was also the highest.
The inhibition effects of pathogen mycelium of watermelon Fusarium wilt in indoor and field control
effect were tested by using endophyte R14,R21,S11and Ha8 introduced from Zhuhai Agriculture Research
Center.The result showed that the inhibitory rate of endophyte R14,R21 and S11 to the fungus of watermelon
Fusarium wilt in indoor were over 80 %;The control effect of,watermelon Fusarium wilt in field were over 60
%,firstly by pouring endophyte Ha8 fermentation broth to root,and then by pouring endophyte R14 and S11
fermentative liquid to root for the second,third and fourth times.
Through investigating the aquatic vegetable resources and productions in 14 counties and cities of
the middle and south area of Anhui Province,62 accessions of aquatic vegetable resources were
collected,including 18 accessions wild resources,44 accessions of local cultivars.Some special local
cultivars such as‘Xuehugong’ou’,‘Tongchengshuiqin’,‘Jixishuiyu’and‘Lujingqinya’,etc.was
collected.And cultivation and production of aquatic vegetables in middle and south areas of Anhui Province
was also investigated.According to the result of investigation,suggestion was put forward that water dropwort
cultivated in our country should be two kinds.One is Oenanthe javanica(BL.)DC.,and the other is Oenanthe
sinensis Dunn.
‘Jinqiu 65’is a new earlymaturity autumn Chinese cabbage F1 hybrid developed by crossing self
-incompatible lines H38 with H218.Its growth period is about 65 days.The plant is 45 cm in height,55 cm in
width.Its leaf head is 37 cm in height,14 cm in diameter and 2.5 kg in weight.The plant type is compact and
with dark green leaves,moderate leaf-wrinkle and light green petiole.The leaf head is overlapping on top.It
has higher resistance to downy mildew,soft rot and virus than‘Qiulu 60’.It can yield about 60 t·hm-2.
‘Hangza No.1’is a new early maturity tomato F1 hybrid,developed by crossing 8947-1-2-2-3-1 as
male parent with 9905-1-2-1-1-1 as female parent.It is about 113 days from sowing to harvesting.It is a
variety with indeterminate growth and red color fruit.Its average single fruit weight is about 200 g.Its
fruit hardness is 0.97 kg·cm-2.It is good for storage and transportation.It has excellent quality.Its VC
content is 159.0 mg·kg-1 and licopene content is 32.4 mg·kg-1.The soluble solid content is 5.03 %.It is
resistant to leaf mold.It can yield about 90 t·hm-2.It is suitable for plastic shed cultivation in spring or
in protected field in early spring in the Yangtze River Valley.
‘Beiyan No.1’takes 05B-91 as female parent,and 05B-93 as male parent.It is a F1 hybrid tomato
with hard flesh and big fruit.It belongs to boundless growth type with middle early maturity.Its growing
period is about 106 days.Its matured fruit is red in color and of flat round shape.Its soluble solid content
is 4.8 %,VC content is 114.4 mg·kg-1,soluble general sugar content is 2.7 %.It is resistant to leaf mould
and ToMV.Its fruit hardness is 0.84 kg·cm-2.It is tolerant to storage and transportation.The average single
fruit weight is 192.7 g.Its yield is about 90 t·hm-2.It can be cultivated in open or protected fields all
over China.
‘Yinzhu’is a new mid-maturity F1 hybrid of muskmelon developed by crossing C70(03TM25-4)as female
parent and C69 as male parent.In open field,its whole growing period lasts about 98 days and its fruit
development period is about 50 days.It has strong growth vigor.It is resistance to leaf blight and downy
mildew,mid-resistance to powdery mildew.The fruit shape index is about 1.1.It has long round fruit with
milky-white skin,with or without sparsity net.The fruit flesh is white-green and about 3.5 cm in
thickness.The soluble solid content is 15 %-17 %.Its firm rind is suitable for transportation.The fruit
weight is about 1.1 kg.The yield is about 31.5 t·hm-2.It is suitable for cultivation open or protected field
in Northwestern China.It extension area is about 13 000 hm2.