1Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences,Hohhot 010031,Inner Mongolia,China;2College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
Abstract:The process of tomato breeding is facilitated by the use of materials with a high level of genetic diversity,which can be used to develop new varieties with specific target traits.In this study,16 quality traits and 8 quantitative traits of 96 processed tomato germplasm resources were evaluated using a mutation level approach,and analysed with insertion/deletion(InDel)markers.The results demonstrated that the phenotypic traits of the tested materials exhibited a high degree of genetic diversity.The highest Shannon-Wiener diversity index for quality traits was observed for fruit shape(H′ = 1.66).The genetic diversity index(H’)of quantitative traits exhibited a range of values,from 1.67 to 4.56.The highest values were observed for fruit weight(4.56)and pulp thickness(4.56).The average number of effective alleles(Ne)detected by 48 pairs of InDel markers was 1.710,with a polymorphism information content(PIC)ranging from 0.041 to 0.540,with an average of 0.330.InDel labeling cluster analysis revealed that 96 materials could be divided into three categories.Group 1,which contained 39 resources,exhibited strong growth and fruit hardness.The second group contains a single resource,and all characters are neutral.The third group,comprising 56 resources,was distinguished by short plants and low hardness.Therefore,parental materials can be selected from the aforementioned groups based on target traits.In conclusion,the experiment provides a more accurate and comprehensive scientific basis for the utilisation and breeding of processed tomato germplasm resources.