Abstract:Three cold-tolerant lines(NY-2, JY-2 and Hot-1)and one cold-sensitive cultivar(Jinyan No.4)of cucumber(Cucumis sativus L.)were treated with temperatures of 28 ℃/18 ℃(day/night)or 28 ℃/12 ℃ with or without pollination. Marketable yield and 3 yield components(node number, percentage of nodes with female flowers and fruit setting rate)were recorded. The results show that 3 cold-tolerant genotypes rely on different mechanisms to keep relatively higher yield than‘Jinyan No.4’under cold stress. Comparing to‘Jinyan No.4’, JY-2 has more node numbers, NY-2 has more cold-tolerant pollens, and Hot-1 has higher parthenocarpic ability. Cold tolerance of NY-2 pollens is higher than that of‘Jinyan No.4’and JY-2. Under low temperature, JY-2 and Hot-1 plants pollinated with NY-2 pollens can produce higher yields than those pollinated with JY-2 pollens.