Studies on Edible Lily in vitro Conservation Technique by Slow-growth Method
ZHANG Yu-qin1, FENG Jian-jun2, ZHAO Jing-fang3
1College of Agronomy, Inner Mongolia University for Nationalities, Tongliao 028043, Inner Mongolia, China; 2Agriculture Bureau of Xinganmeng, Keyouzhongqi 029400, Inner Mongolia, China; 3Agricultural Technology Extension Center of Tuquan County, Xinganmeng 137500, Inner Mongolia, China
Abstract：In order to establish conservation system for edible lily slow-growth germplasm resources, the test-tube plantlets gained from micro-propagation were inoculated in 12 different culture media and conserved under 10 ℃ and 25 ℃ for 6 months, respectively, so as to observe the growth of test-tube plantlets under different treatments. The results showed that the low mannitol concentration has poor control affect on the growth of test-tube plantlets, while the high concentration severely affect the plantlets growth. The suitable mannitol concentration was 20 g•L-1 for conserving edible lily plantlets with slow-growth. Sucrose has restrain effect on the growth of test-tube plantlet, 60 g•L-1 sucrose is the suitable media. 20 g•L-1 mannitol+60 g•L-1 sucrose is the best treatment for slow growth conservation of edible lily test-tube plantlets. Their survival rate can reach 100 %, and the rate of bulb formation is high. 10 ℃ can keep the plantlet grow slowly and promote the bulb formation for 6 months conservation without subculture, while under 25 ℃ the rate of bulb formation is reduced, and the medium loss is serious. The plantlets must be preserved with subculture.
