Effects of Feeding Different Aquatic Vegetables on Sensitivity to Insecticides of Spodoptera litura（Lepidoptera: Noctuidae）
QIN Chun-hua1, 2, KUANG Jing1, YU Ming-hua3, LI Jian-hong1*
1College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 2Nanzhang Branch of Xiangyang Tobacco Corporation, Xiangfan 441500, Hubei, China; 3Meichuan Town Agricultural Technology Extension Centre of Wuxue City, Wuxue 435400, Hubei, China
Abstract：In order to test Spodoptera litura fed by different aquatic vegetables on sensitivity of 5 different insecticides, the activity of detoxifying enzymes was assayed by leaf dipping method. The results indicated that the sensitivities of S. litura to chlorfluazuron, chlorpyrifos, indoxacarb, chlorfenapyr and emamectin-benzoate were different when feeding on different aquatic vegetables. Activity of detoxifcation enzymes of S. litura was different when feeding on different aquatic vegetables, and sequence of the activity of CarE was Nelumbo nucifera＞artificial diet＞Oenanthe stolonifera＞Colocasia esculenta. The order of specific activity of AChE was C. esculenta＞artificial diet＞N. nucifera＞O. stolonifera. The activity of GSTs was artificial diet＞O. stolonifera＞N. nucifera＞C. esculenta. The activity of MFO was O. stolonifera＞C. esculenta＞N. nucifera＞artificial diet. It demonstrated that high detoxification of enzymes was related to different sensitivities to insecticides of S. litara, when feeding on different aquatic vegetables.
