1College of Plant Protection，Qindao Agricultural University，Qingdao 266109，Shandong，China|2Plant Protection Station of Qindao，Qingdao 266100，Shandong，China
Abstract：Strain degeneration is a key problem occurred in Cordyceps militaris（L.）link cultivation．In this study，four rejuvenation methods including isolation of ascospores，conidia，silkworm chrysalis and substrate mycelium were used．And the growth rate of mycelia，color changing speed and formation of stroma were investigated．The results indicated that two methods including isolation of ascospores and silkworm chrysalis had remarkable effects on rejuvenation．
