Expression of EGFP Gene Controlled by Rape Seed-specific Napin Promoter in Transgenic Mustard
ZOU Min，TANG Jia-jia，SONG Hong-yuan*
Key Laboratory of Horticulture Science for Southern Mountains Regions，Ministry of Education，Chongqing key Laboratory of Olericulture，College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400715，China
Abstract：In order to study the function of rape seed-specific Napin promoter in mustard〔Brassica juncea（L.）Czern. et Coss.〕，Napin promoter was fused to the 5′-upstream of an Enhanced Green Fluorescent Protein（EGFP）reporter gene and introduced into mustard by Agrobacterium-mediated transformation．The expression of EGFP in transgenic mustard seeds and their T1 seedlings showed that EGFP gene was expressed in seed coat，cotyledon and radicle of seeds and that the fluorescent intensity in all there parts were strengthened gradually with transgenic seed maturation．And the fluorescent intensity were weaken gradually in root，cotyledon and hypocotyl with the seed germination．All these results indicated that the rape Napin promoter could initiate downstream gene expression in mustard specifically．
