CAPS Marker and Detection of Resistant Gene Ty-1 in Tomato
LI Hai-tao1，2，DU Yu-li2，ZHANG Zi-jun1*，QU Tong1，ZOU Qing-dao1*
1Institute of Vegetable，Liaoning Academy of Agricultural Sciences，Shenyang 110161，Liaoning，China|2College of Horticulture，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
Abstract：In order to obtain molecular marker of resistant gene Ty-1 in tomato（Lycopersicon esculentum Mill.），3 resistant homozygous lines（Ty-1/Ty-1）and 3 homozygous susceptible lines（ty-1/ty-1）were amplified by specific primer．Both resistant and susceptible lines produced about 400 bp PCR fragment．After digestion with enzyme Rsa I，susceptible genotypes and heterozygous resistant genotypes could produce fragment about 350，50 bp and 400，350，50 bp，respectively．While the resistant genotypes still presented 400 bp fragment．This marker could distinguish resistant and susceptible lines，was a co-dominant marker tightly linked to Ty-1 gene．33 F1 hybrids were detected using this marker，and the results showed that 20 hybrids contained Ty-1 resistant gene and 13 hybrids did not．This marker was also used to detect 24 important inbred lines and all of them did not contain Ty-1 gene．The repeated verification proved that Ty-1 resistant gene could be identified by this marker．
