Abstract：The pot experiment was conducted by using the bio-organic fertilizer（BOF）made
by mixing the mature compost with functional bacteria to study the effects of different applying quantity
of bio-organic fertilizer on controlling bitter gourd（Momordica charantia L.） Fusarium wilt
and on soil microorganisms and enzyme activities．The results indicated that the occurrence of bitter
gourd Fusarium wilt was decreased significantly by applying BOF．The disease-control effects
of the 3 BOF treatments reached 22.21%，38.91% and 44.45%，respectively，when the diseasecontrol
effect of CF was 0. With the same effective nutrient，the plant stem diameter，leaf length
and leaf width，and plant dry weight under BOF2 treatment were significantly higher than that of CF
treatment，with remarkable growth stimulating effect．The number of bacterium and actinomycesin soil were increased by BOF，while the amount of fungi and Fusarium oxysporum in soil were reduced
evidently．Application of BOF could increase the activities of soil urease，phosphatase and
invertase，which were higher than those of CF treatment．But the change of catalase was uncertain．
The results showed that BOF could improve soil microbial flora and enhance soil enzyme activity，improve
soil environment condition，and prevent the occurrence of Fusarium wilt.
