Effect Evaluation on Rapid Enzyme Inhibiting Method to Detect Pesticide
Residues in Vegetable
YE Xue-zhu，WANG Qiang，ZHAO Shou-ping，ZHANG Hu，WANG Xiang-yun，ZH AO Yan-shen
Institute of Quality and Standard for Agro-Products， Zhejiang Academy of Agriculture Sciences， MOA Key Lab for
Pesticide Residue Detection， Hangzhou 310021， Zhejiang， China
Abstract：This paper evaluated the effect of daily used enzyme inhibition method to rapid detect
pesticide residues in vegetables，by chromatography quantitative detection．The results showed that
there were bigger differences in sensitivities of different pesticides using this method，among them sensitivity
of carbofuran was the best，and the lowest can reach 0.003 mg·kg-1，followed by methomyl and
carbaryl．But the sensitivities of chlorpyrifos and triazophos，which were often detected in present vegetables，
were not high．The rapid detection had false-positive and false-negative problems．The falsepositive
rates of vegetable products were 7.1%-26.2%，while the false-negative rates to test the pesticide
chlorpyrifos for vegetables sample were generally high．Therefore，the method of enzyme inhibition has
played certain role in rapid controlling high toxic vegetables，but as law enforcement detection，further
research is essential to improve the enzyme reagent quality and to study the pretreatment method.
