In order to identify the Phytophthora capsici stress induced genes of pepper,a double-hybrid cDNA library of Phytophthora capsici infected pepper leaves was constructed using SMART technology. The results showed that the cDNA library contained 3.6×106 independent clones,and the recombination rate was 88%.Insert fragments ranged from 300 bp to 2 000 bp,and the average length of the library was about 800 bp. These data demonstrate that the library is qualified,and able to provide important genetic resources for molecular breeding.
周志国,明月梅. SMART 技术构建的辣椒疫霉菌与辣椒互作AD-cDNA 文库[J]. 中国蔬菜, 2015, 1(11): 51-54.
ZHOU Zhi-guo,MING Yue-mei. Construction of AD-cDNA Library for Interaction of Phytophthora capsici and Pepper in SMART Technology. China Vegetables, 2015, 1(11): 51-54.