In order to ascertain the new resource utilizating way of cassava starch waste for environmental protection，this paper studied the effect of cassava starch anaerobic fermentation liquid（ CSAFL）on pumpkin growth，yield and fruit quality，taking‘ Mibennangua’ as experimental material．The results showed that applying CSAFL could significantly promote the vegetative and reproductive growth，increase pumpkin yield and improve its fruit quality． Using CSAFL could also remarkably promote the growth of pumpkin main stem and leaves，increase leaf chlorophyll content. Besides，its fruit setting number per single plant，single fruit weight and yield were higher than that of the contrast by over 50%. Its fruit size，fruit shape index，contents of soluble solid，soluble sugar，soluble protein，starch and VC were significantly or very significantly increased than that of  the contrast．Therefore，CSAFL can be used as additional fertilizer in pumpkin production，so as to realize the dual purposes of turning waste into wealth，and improving pumpkin yield and quality.