The Bt gene，Cry2Aa2，were transferred into Chinese kale（Brassica alboglabra L. H.
Bailey）by biolistic and agrobacterium-mediated transformation methods and these 2 transformation systems were
optimized and compared．High transformation rate was obtained by bombarding Chinese kale callus with a distance
of 6 cm and for one time．The transformation efficiency was 0.43%. The suitable procedure for agrobacteriummediated
transformation method in Chinese kale was using hypocotyl as material，pre-culture for 2 days，infected
by agrobacterium for 10 minutes，co-culture for 2 days，antibacterial-culture for 5 days and then screened in
selective culture with 5 mg·L-1 kanamycin．The transformation efficiency was 0.37%．Identified by PCR，PCRSouthern
blot analyses，the target gene were transferred into the Chinese kale by both biolistic and agrobacteriummediated
transformation methods．Insect resistance identification proved that the positive transgenic Chinese kale
plants had stronger resistance to Mamestra brassicae larva than the non-transgenic plants.
