Identification and Preliminary Application of Antagonistic Strains Against Potato Fungal Diseases
YANG Fan 1,2,ZHANG Fei-yan2,SUN Jin-chong2,LIU Shuang3,LIU Hong-wei2,ZHANG Li-ping 2*,HU Bin 1*
1Hebei University of Technology,Tianjin 300130,China;2Institute of Biology,Hebei Academy of Science,Shijiazhuang
050081,Hebei,China;3Hebei Animal Husbandry Station,Shijiazhuang 071000,Hebei,China
Aiming at the issues of serious occurrence of potato fungal diseases,mixed occurrence of
various diseases,lacking effective control methods,this paper classified and identified highly effective
antagonistic strain BA-26 screened out at earlier stage,studied the stability of its extracellular metabolites,
and clarified its role on preventing disease and promoting growth.BA-26 strain was identified as Bacillus
amyloliquefaciens,through analyzing morphologic characteristics,physiological and biochemical properties,
and 16S rDNA sequence analysis.This strain showed strong antagonistic effects on 8 potato pathogenic
fungus,and its inhibition ratio against Alternaria solani,Fusarium solani and Fusarium avenaceum was
over 80%.After treating the extracellular metabolites by 100 ℃ for 30 min,UV irradiation for 8 hours,pH
2-10 acid-base,and protease for an hour,its relative antibacterial activities were all maintained above 80%.
Laboratory test of bacteriostatic activity showed that the inhibition ratio of BA-26 filtrate against Fusarium
solani was 91.3%.In pot experiment,the potato root length,fibrous root number,plant height and
chlorophyll content in leaves were significantly increased by 32.07%,111.94%,48.0% and 19.6%,
respectively by applying BA-26 fermentation broth with 20 mL concentration of 4.0×109 cfu·mL-1 per pot.
The results indicated that B. amyloliquefaciens BA-26 had broad bacteriostasis spectrum,strong stability of
extracellular metabolites,and good disease prevention and growth promotion effect,having great potential for
developing biocontrol agent exclusively for potato.