（Environment and Plant Protection Institute，Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Pests
Comprehensive Governance for Tropical Crops，Ministry of Agriculture and Rural Affairs，Haikou 571101，Hainan，China）
利用枯草芽孢杆菌BLG010 对苦瓜白粉病进行防治试验，同时为了探明苦瓜白粉病的病程相关物质，利用气相色谱-质谱联用技术分析白粉病菌接种处理、白粉病菌+BLG010 接种处理和蒸馏水喷施处理后8 d 和15 d 时苦瓜叶片的代谢物质差异。试验结果表明，BLG010 对苦瓜白粉病的防治效果高达89.49%；苦瓜感染白粉病菌后，D- 葡萄糖、延胡索酸和肌醇半乳糖苷含量在8 d 和15 d 均上调表达，而甘氨酸、甘油酸、L- 谷氨酸和咖啡酸则表现为下调。这7 种代谢物质的含量变化与苦瓜白粉病病程相关，施用BLG010 可以降低苦瓜白粉病病情指数，避免这7 种代谢物质含量的异常变化。
This paper conducted experiment on controlling bitter gourd powdery mildew using Bacillus
subtilis（BLG010）．And in order to find out the pathogenic process-related substances，the paper analyzed
the differences in metabolic substances of bitter gourd leaves 8 days and 15 days after pathogen inoculation
treatment，pathogen+BLG010 inoculation treatment and distilled water spraying treatment by GC-MS
methodology．The results showed that the control effect of BLG010 on bitter gourd powdery mildew was as
high as 89.49%．After pathogen infection，the contents of D-glucose，fumaric acid and galactinol on 8 days
and 15 days were all up-regulated．While，the contents of glycine，glyceric acid，L-glutamic acid and caffeic
acid were down-regulated．Contents of these 7 metabolic substances were related to the pathogenic process of
bitter gourd powdery mildew．Application of BLG010 can decrease the disease index，and avoid the abnormal
changes in contents of these 7 metabolic substances．
