（1Key Laboratory of Protected Vegetable Germplasm Innovation，Ministry of Agriculture and Rural Affairs，Key Laboratory of
Protected Vegetable Molecular Breeding，Shandong Province，Vegetable Engineering Technology Research Center of Shandong
Province，Shandong Vegetable Seed Industry Group Co.，Ltd.，Shandong Shouguang Vegetable Industry Group Co.，Ltd．
of Shouguang Province，Shouguang 262700，Shandong，China；2Shouguang Agricultural Technology Center，Shouguang
262700，Shandong，China；3Shandong Shouguang Ouyate Vegetable Co.，Ltd.，Shouguang 262700，Shandong，China；4Key
Laboratory of Biochemistry and Molecular Biology，Colleges and Universities of Shandong Province，College of Biological and
Agricultural Engineering，Weifang University，Weifang 261061，Shandong，China；5Institute of Vegetables and Flowers，
Chinese Academy of Agricultural Sciences，Beijing 100081，China；6Institute of Genetics and Developmental Biology，Chinese
Academy of Sciences，Beijing 100101，China）
Abstract：Taking advantage of the CAPS marker C2-25 closely linked to the resistant gene Frl to tomato
Fusarium crown and root rot，this paper developed and designed new SCAR markers to quickly detect the
dominant resistant gene Frl．Using C2-25 as the primer，this experiment conducted PCR among the 25 known
phenotypic tomato material through monoclonal sequencing，of which 19 homozygous resistant material all
containing one same resistant sequence，4 homozygous susceptible material all containing one same susceptible
sequence，both 2 heterozyous resistant material containing the above mentioned resistant and susceptible
sequences．According to SNP differences between the resistant and susceptible sequences，we designed
primers and finally found out primer combination R-6F/2R，S-3F/4R linked to Frl gene and produced 370 bp
resistant fragment and 520 bp susceptible fragment．Testing the primer combination among 500 F2 plants，
the genotype of 473 plants were consistent with their phenotype，meaning the accuracy rate was 94.6%．This
SCAR marker was characterized by high accuracy，low cost and simple operation．Therefor，it can be used in
gene Frl marker assisted breeding．
