1Plant Science and Technology College，Beijing University of Agriculture，Beijing 102206，China；2Research Center for
Vegetables，Beijing Academy of Agriculture and Forestry Sciences，Key Laboratory of Biology and Genetic Improvement of
Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs，Beijing Vegetable Germplasm Improvement
Lab，Beijing 100097，China
Abstract：In order to explore the regulatory mechanism of low temperature and weak light environment
on sugar accumulation of watermelon，this experiment analyzed the transcriptome data of mutant of alkaline
alpha-galactosidase gene aga2，and found that there were 686 up-regulated genes and 990 down-regulated
genes in aga2 mutant fruits under low temperature and weak light．By GO and KEGG analysis，it was further
found that the up-regulated expression of transcription factors：MYB，NAC and WRKY etc. could resist low
temperature and weak light．While，the down-regulated expression of genes related to sugar metabolism
like galactinol synthase（Cla009222），alpha-galactosidase（Cla022883、Cla019238）etc. induced by
low temperature and weak light may be the main reason for the decrease of sugar content in mutant fruit．
By analyzing the transcriptome data，this experiment excavated the genes related to sugar accumulation in
watermelon responding to low temperature and weak light，and laid the foundation for regulation network of
sugar metabolism in watermelon responding to low temperature and weak light．
