Abstract：Identification on strawberry root rot pathogen was conducted by morphology and molecular biology technique．Tissue culture，pathogen isolation and purification of sicken strawberry roots in strawberry breeding nursery was carried out．The pathogen strain was initially diagnosed as Ilyonectria pathogen by traditional morphological observation．The ZJ16 strain was initially identified by universal primers ITS1/ITS4．It was belonging to the genus Ilyonectria，which could not be identified as species．The specific gene EF-1α and Histone H3 primer sequence were selected and analyzed by PCR amplification．Homology comparison between sequencing result of waiting tested strain and related strain EF-1α and Histone H3 sequence in GenBank were conducted．The homology of waiting tested strain and Ilyonectria novozelandica（CBS 112593）were all 100%．It was confirmed that strawberry root rot pathogen in Xinjiang region was identified as Ilyonectria novozelandica，which was a new recorded species in China．
