1Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，Henan，China；2Tibet Agricultural and Animal Husbandry College，Linzhi 860000，Tibet，China
Abstract：A total of 183 endophytic bacteria strains and 55 rhizosphere bacteria strains were isolated by tissue separation and dilution separation methods from strawberry root，stem，leaf and rhizosphere soil．Among them，9 strains showing broad-spectrum antagonistic effects against 4 strawberry fungal pathogens，including Pilidium concavum，Botrytis cinerea，Fusarium oxysporum and Alternaria alternate．Combining the results of morphological trait，physiology and biochemistry character，and molecular identification，strains G3-23，J3-20 and G3-29 were identified as Bacillus velezensis；J6-1，J1-4，G2-12，G3-K2，and J1-11 were identified as B. subtilis；G3-20 was identified as B. halotolerans；and G3-17 was identified as B. cereus．Strain G3-23 could secrete protease and cellulase，and had a good control effect on strawberry tan-brown leaf spot caused by P. concavum．Besides，this strain could successfully colonize on strawberry leaf，showing a good biocontrol potential．
王卓妮1，2☆，覃艮红1☆，王 丽1，侯 珲1，袁洪波1，周厚成1，巩文峰2*，涂洪涛1*. 草莓病害拮抗细菌的筛选及其对草莓褐色叶斑病的防效[J]. 中国蔬菜, 2023, 1(2): 63-71.
WANG Zhuoni1，2 ☆，QIN Genhong1 ☆，WANG Li1，HOU Hui1，YUAN Hongbo1，ZHOU Houcheng1，GONG Wenfeng2*，TU Hongtao1*. Screening of Antagonistic Bacteria from Strawberry and Their Biocontrol Effect against Tan-brown Leaf Spot of Strawberry. China Vegetables, 2023, 1(2): 63-71.
