Studies on Pathogenicity and Isolate Identification of Pathogen Causing Chinese Cabbage Black Spot in Qingdao Region
SONG Yumeng，LIU Wenjie，WANG Min，YUAN Zhonglin，LUO Lan*
（Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management，College of Plant Health and Medicine，Qingdao Agricultural University，Qingdao 266109，Shandong，China）
Abstract：In order to clarify the kind of pathogen causing Chinese cabbage black spot in Qingdao region of Shandong Province，this experiment collected 18 accessions of sickened Chinese cabbage samples from Qingdao City Jiaozhou，Laixi and Jimo，in 2019—2020；isolated pathogenic fungi by tissue separation method；conducted classification and identification through morphological observation and molecular biological techniques；and carried out pathogenicity test according to Koch’s rule．The results showed that 21 strains were isolated from the sickened Chinese cabbage samples collected from Qingdao region，belonging to Alternaria brassicae，A. japonica and A. alternate，separately．Among them，A. alternate was the dominant pathogen with detection rate of 76.2%．But A. brassica was the most virulent with a disease index of 48.15．
宋雨萌，刘文杰，王敏，袁忠林，罗兰*. 青岛地区大白菜黑斑病病原菌分离鉴定与致病性研究[J]. 中国蔬菜, 2023, 1(4): 57-63.
SONG Yumeng，LIU Wenjie，WANG Min，YUAN Zhonglin，LUO Lan*. Studies on Pathogenicity and Isolate Identification of Pathogen Causing Chinese Cabbage Black Spot in Qingdao Region. China Vegetables, 2023, 1(4): 57-63.
