Screening Identification and Bio-control Effect of Antagonistic Fungus against Pepper Root Rot
LI Shujiang，ZHANG Yunxia，LIU Yu，ZHOU Chuangchuang，ZHANG Zhiqin，FENG Dao，YANG Youlian，WU Di，ZHANG Xiaoyong
1 School of Biological Sciences and Technology，Liupanshui Normal College，Liupanshui 553004，Guizhou，China；2 Liupanshui City Academy of Agricultural Sciences，Liupanshui 553000，Guizhou，China
Abstract：In order to obtain high efficient bio-control fungus for pepper root rot，and isolate fungi strain from rhizosphere soil of healthy pepper plants，this paper conducted confrontation culture with Fusarium solani；and screened the antagonistic strain M20210527-2 with 79.29% inhibition rate on Fusarium solanum．Combined with morphology and ITS molecular biology analysis，strain M20210527-2 was identified as Trichoderma harzianum．Strain M20210527-2 could quickly compete for growth space and secrete antifungal metabolites for effective inhibition on Fusarium solani growth by hyperparasitism.Strain M20210527-2 treatment could relatively increase the control efficiency on pepper root rot as high as 94.25% and also significantly reduce its incidence and severity. Studies on culture conditions indicated that the optimum growth temperature for strain M20210527-2 was 25 ℃；optimums pH was 6-7；optimum growth carbon sources were sucrose，glucose，and starch；optimum growth nitrogen sources were peptone，yeast powder and beef extract．
