Spatiotemporal Expression Analysis of Odorant Receptor Genes of BodoOR71 and BodoOR72 in Bradysia odoriphaga
（1Institute of Insect，College of Agriculture，Yangtze University，Jingzhou 434025，Hubei，China；2Institute of Agricultural Quality Standards and Testing Technology Research，Hubei Academy of Agricultural Sciences，Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products，Wuhan 430064，Hubei，China）
Abstract：
Abstract：Based on the transcriptome data of Bradysia odoriphaga，the full-length cDNA sequencesof BodoOR71 and BodoOR72 were cloned by RT-PCR，sequence as well as structure analysis were done bybioinformatic tools，and comparison between expression levels of these genes in different developmental stagesand adult tissues were done．The sequence analysis showed that BodoOR71 had an open reading frame（ORF）of 759 bp，encoding a protein of 253 amino acids with four transmembrane domains，and BodoOR72 had anORF of 954 bp，encoding a protein of 318 amino acids with six transmembrane domains．The phylogeneticanalysis indicated that BodoOR71 and BodoOR72 were closely related to other Diptera insects，which can beclustered into one clade．The temporal and spatial expression pattern showed that BodoOR71 was expressed at different developmental stages except males and highly expressed in females．The expression level of BodoOR71was significantly higher in female antennae than other tissues and was barely detected in males．BodoOR72 wasexpressed at different developmental stages and highly expressed in males．The expression level of BodoOR72stayed low in all tissues of female while turned significantly higher in male antennae than other tissues．Toconclude，BodoOR71 and BodoOR72 may involve in the olfactory recognition of B. odoriphaga．
